{"id":2425,"date":"2019-05-09T13:47:07","date_gmt":"2019-05-09T11:47:07","guid":{"rendered":"https:\/\/www.kromidas.de\/hplc\/kurse\/hplc-basiskurs\/"},"modified":"2024-09-13T16:33:08","modified_gmt":"2024-09-13T14:33:08","slug":"hplc-basic-course","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/hplc-basic-course\/","title":{"rendered":"HPLC Basic Course"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none”][vc_column column_padding=”padding-2-percent” column_padding_position=”top-bottom” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

HPLC Basics for Beginners incl. Troubleshooting<\/span><\/h3>\n[\/vc_column_text][vc_column_text]\n

Training overview<\/strong><\/h3>\n[\/vc_column_text][vc_column_text]This 2-days-course has several targets: First of all it is suitable for beginners in HPLC; secondly, HPLC-users having worked an HPLC in the routine so far, get some background for understanding their daily work better. This interactive training includes specific exercises and tutorials to ensure that the basic knowledge concerning HPLC is learnt profoundly. A further topic is how to recognise whether validated methods are robust or even not. The participants learn to detect and understand simple variances and are able to derive the right action for their daily work[\/vc_column_text][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

Training content<\/h3>\n[\/vc_column_text][vc_column_text]\n
    \n
  • The principle and the target of HPLC \u2013 qualitative and quantitative<\/li>\n
  • \u00a0Influence of column length, flow, column packing, mobile phase, temperature etc. on the chromatogram<\/li>\n
  • \u00a0RP-chromatography, principle and legalities<\/li>\n
  • What does: \u201cSupersil\u201d, ODS II e, 100, 5, 125 x 4,6 mean?<\/li>\n
  • The HPLC-apparatus \u2013 what do the single devices effect?<\/li>\n
  • \u201cSimple\u201d but important hand grips like \u201chow to flush and store the column?\u201d or \u201cdoes the ghost peak come from the sample, the eluent or the needle?\u201c<\/li>\n
  • Troubleshooting on the basis of typical symptoms<\/li>\n
  • What is \u201ctabu\u201d managing a HPLC-system and which actions have to be considered?<\/li>\n
  • Tutorials and exercises concerning deviations in the practical experience compared to the theoretical forecast<\/li>\n
  • Methods which are validated, long term used but not robust: A critical glance on the method description<\/li>\n<\/ul>\n[\/vc_column_text][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

    Target audience<\/h3>\n[\/vc_column_text][vc_column_text]\n
      \n
    • Beginners and HPLC-users with little knowledge<\/li>\n<\/ul>\n[\/vc_column_text][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

      Training objectives<\/h3>\n[\/vc_column_text][vc_column_text]\n
        \n
      • With the help of the learned aspects concerning HPLC, the participants can carry out HPLC-seperations systematically and competently<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

        [vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none”][vc_column column_padding=”padding-2-percent” column_padding_position=”top-bottom” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text] HPLC Basics for Beginners incl….<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":97,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2425","page","type-page","status-publish"],"yoast_head":"\nHPLC Basic Course - Dr. Stavros Kromidas<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/hplc-basic-course\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HPLC Basic Course - 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