{"id":2426,"date":"2019-05-09T13:47:07","date_gmt":"2019-05-09T11:47:07","guid":{"rendered":"https:\/\/www.kromidas.de\/hplc\/kurse\/hplc-fortgeschrittenenkurs\/"},"modified":"2024-12-09T18:48:38","modified_gmt":"2024-12-09T17:48:38","slug":"advanced-hplc","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/advanced-hplc\/","title":{"rendered":"Advanced HPLC"},"content":{"rendered":"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent” column_padding_position=”top-right” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

HPLC for Advanced Users incl. Troubleshooting<\/span><\/h3>\n[\/vc_column_text][vc_column_text]\n

Training overview<\/h3>\n[\/vc_column_text][vc_column_text]This training has been established for participants that are already experienced in HPLC. Focus of the training is to go into details on the aspects that are important for you to manage your daily requirements: Pragmatic approaches for quick optimization, understanding of the background, troubleshooting. The \u201cwhat\u201d and \u201cwhy\u201d will be trained in groups on the basis of real cases. A classic course for years now\u2026[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent” column_padding_position=”right” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

Training content<\/h3>\n[\/vc_column_text][vc_column_text]\n
    \n
  • Capacity, selectivity or efficiency \u2013 what do I really need for a good resolution? their influence on the separation<\/li>\n
  • Stationary phases: column types, description for use, \u201cbest\u201d column suitable for your individual separation problem, new stationary phases \u2013 pros and cons<\/li>\n
  • Gradient elution \u2013 a step towards optimization?<\/li>\n
  • Method development \u2013 recommendations for quick solutions<\/li>\n
  • Pros and cons for UHPLC, Core Shell, Monoliths, Fast LC, Sub 2 \u00b5m particles etc.<\/li>\n
  • Peak shape \u2013 an indicator for errors<\/li>\n
  • Chemical tailing; the challenge of separating polar substances<\/li>\n
  • What information can be gathered from a chromatogram \u2013 learning to \u201cread\u201d the chromatogram!<\/li>\n
  • How to find\u00a0and<\/em>\u00a0eliminate errors<\/li>\n
  • Tests and \u201chow-to-do\u201d-lists for daily lab-work<\/li>\n<\/ul>\n[\/vc_column_text][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

    Target audience<\/h3>\n[\/vc_column_text][vc_column_text]\n
      \n
    • Advanced HPLC-users, minimum experience: 1 year<\/li>\n<\/ul>\n[\/vc_column_text][divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

      Training objectives<\/h3>\n[\/vc_column_text][vc_column_text]\n
        \n
      • Getting familiar with a systematic and economic approach within HPLC in your everyday work<\/li>\n<\/ul>\n[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=”full_width_background” full_screen_row_position=”middle” equal_height=”yes” content_placement=”middle” column_margin=”default” bg_color=”#ee9d34″ scene_position=”center” text_color=”light” text_align=”left” top_padding=”40″ bottom_padding=”30″ overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/2″ tablet_width_inherit=”default” tablet_text_alignment=”center” phone_text_alignment=”center” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_custom_heading text=”Interest in this course?” font_container=”tag:h3|font_size:35|text_align:left|line_height:42px” use_theme_fonts=”yes” css=”.vc_custom_1583745366302{padding-left: 25px !important;}”][vc_column_text css=”.vc_custom_1583745176710{padding-top: -10px !important;padding-left: 25px !important;}”]Get in touch with me.[\/vc_column_text][\/vc_column][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/2″ tablet_width_inherit=”default” tablet_text_alignment=”center” phone_text_alignment=”center” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”right” css=”.vc_custom_1550215597768{padding-right: 25px !important;}”][vc_column_inner column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][nectar_btn size=”large” button_style=”see-through-3d” color_override=”#ffffff” url=”https:\/\/www.kromidas.de\/kontakt\/” text=”Send an e-mail”][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

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