{"id":2429,"date":"2019-05-09T13:47:07","date_gmt":"2019-05-09T11:47:07","guid":{"rendered":"https:\/\/www.kromidas.de\/hplc\/kurse\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/"},"modified":"2026-04-02T08:32:52","modified_gmt":"2026-04-02T06:32:52","slug":"optimierung-in-der-hplc-effizient-und-zielgerichtet","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/","title":{"rendered":"Method development and optimization of HPLC"},"content":{"rendered":"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent” column_padding_position=”right” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n
Method development and optimization of HPLC for experienced users<\/span><\/h6>\n[\/vc_column_text][vc_column_text]\n

Training overview<\/h3>\n[\/vc_column_text][vc_column_text]This 1-day-course has a clear target: The knowledge of tools for a fast and competent HPLC-optimisation. A common situation: Experienced users know the most parameters of influence and tricks for optimisation in HPLC. Sometimes something is missing, but it is important because of time-saving: approved strategies for systematically planning and efficient carry out of optimizing chromatography.
\nExactly for this case we have developed this course. We give tools and hints to the attendants for an effective realization of several optimisation strategies.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

Training content<\/h3>\n[\/vc_column_text]
\n
    \n
  • Apparative and chromatographic terms for: better resolution, faster chromatography, robust methods, bigger toughness, convenient results, rate of costs and time, lower detection limit, \u2026<\/li>\n
  • What kind of chromatographic parameters are relevant for what targets?<\/li>\n
  • \u00a0What I do really need: Better selectivity, more plates, larger peak capacity, or \u201cjust\u201d resolution?<\/li>\n
  • Strategies of optimisation for: acids, bases, polar substances, neutral substances, isomers, etc.<\/li>\n
  • HPLC-crux: similar structures, similar polarities, similar molecular weight \u2013
    \nwhat to do?<\/li>\n
  • Unorthodox but successful ways<\/li>\n
  • Fast separations: U(H)PLC, sub2\u03bc, Fused Core etc.- a critical comparison<\/li>\n
  • The \u201c5-step strategy\u201d for selective\u00a0and<\/em>\u00a0robust methods<\/li>\n
  • Check-lists for a quick optimisation during daily lab-work<\/li>\n
  • Discussing special separation-problems<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

    Target audiance<\/h3>\n[\/vc_column_text]
    \n
      \n
    • Advanced users, minimum experience 2 years<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

      Training objectives<\/h3>\n[\/vc_column_text]
      \n
        \n
      • Getting tools to recognize clearly, how to optimize in\u00a0this<\/em>\u00a0case \u2013 why and for what purpose<\/li>\n<\/ul>\n <\/div>[\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

        [vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent”…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":97,"menu_order":11,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2429","page","type-page","status-publish"],"yoast_head":"\nMethod development and optimization of HPLC - Dr. Stavros Kromidas<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Method development and optimization of HPLC - Dr. Stavros Kromidas\" \/>\n<meta property=\"og:description\" content=\"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent”...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/\" \/>\n<meta property=\"og:site_name\" content=\"Dr. Stavros Kromidas\" \/>\n<meta property=\"article:modified_time\" content=\"2026-04-02T06:32:52+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.kromidas.de\/en\/#website\",\"url\":\"https:\/\/www.kromidas.de\/en\/\",\"name\":\"Dr. Stavros Kromidas\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.kromidas.de\/en\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/#webpage\",\"url\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/optimierung-in-der-hplc-effizient-und-zielgerichtet\/\",\"name\":\"Method development and optimization of HPLC - 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