{"id":2430,"date":"2019-05-09T13:47:07","date_gmt":"2019-05-09T11:47:07","guid":{"rendered":"https:\/\/www.kromidas.de\/hplc\/kurse\/die-geheimnisse-des-gradienten-auswahl-optimierung-troubleshooting\/"},"modified":"2024-09-13T16:51:51","modified_gmt":"2024-09-13T14:51:51","slug":"die-geheimnisse-des-gradienten-auswahl-optimierung-troubleshooting","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/die-geheimnisse-des-gradienten-auswahl-optimierung-troubleshooting\/","title":{"rendered":"The \u201cSecrets\u201d of the Gradient \u2013 Selection, Optimization, Troubleshooting"},"content":{"rendered":"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent” column_padding_position=”right” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

The \u201cSecrets\u201d of the Gradient \u2013 Selection, Optimization, Troubleshooting<\/span><\/h3>\n[\/vc_column_text][vc_column_text]\n

Training overview<\/h3>\n[\/vc_column_text][vc_column_text]First we will discuss the criteria of the \u201eright\u201c mobile phase for a given separation problem. We will also talk about alternatives, the role of modifiers and the pH-value. The emphasis of the training adresses gradient elution. The participants will learn light-to-implement optimization tools as well as pitfalls and will hear about gradient myths and strange gradient stories\u2026 With the help of a numerous real examples one can understand the provided to-do-lists that give hints on how to create generic gradients depended on the number of compounds to be separated, the matrix and the analytical target[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

Training content<\/h3>\n[\/vc_column_text]
\n
    \n
  • Methanol or acetonitrile? And: Are organic solvents interchangeable?<\/li>\n
  • I need a pH-value of 3: What should I use \u2013 phosphoric, formic or acetic acid?<\/li>\n
  • Aspects of optimization \u2013 customary\/unusual<\/li>\n
  • A lot of small peaks, related\u00a0and<\/em>\u00a0polar compounds: Stepper gradient and low flow rate or vice versa?<\/li>\n
  • Some particularities of the gradient<\/li>\n
  • What does optimization of the gradient mean (resolution, peak capacity, low LOQ)?<\/li>\n
  • Myths, \u201eunexpected\u201c outcomes, examples<\/li>\n
  • The problem of method transfer (HPLC\/UHPLC, High-\/Low-Pressure-Gradient, different vendors)<\/li>\n
  • Drifting baseline, \u201chumps\u201d, low RSD \u2013 how to proceed<\/li>\n
  • \u201eDo \u0301s and don\u2019t \u0301s\u201c, list of recommendations<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

    Target audience<\/h3>\n[\/vc_column_text]
    \n
      \n
    • Advanced HPLC-users in development departments, minimum experience 2 years<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

      Training objectives<\/h3>\n[\/vc_column_text]
      \n
        \n
      • To gain certainty and ease of handling mobile phase and gradients<\/li>\n<\/ul>\n <\/div>[\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

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