{"id":2701,"date":"2019-05-17T11:59:07","date_gmt":"2019-05-17T09:59:07","guid":{"rendered":"https:\/\/www.kromidas.de\/hplc\/kurse\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/"},"modified":"2023-11-12T10:28:19","modified_gmt":"2023-11-12T09:28:19","slug":"aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/","title":{"rendered":"Advanced troubleshooting in HPLC \u2013 the tricky cases"},"content":{"rendered":"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent” column_padding_position=”right” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” column_link_target=”_self” width=”1\/1″ tablet_width_inherit=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n
Advanced troubleshooting in HPLC \u2013 the tricky cases<\/span><\/h6>\n[\/vc_column_text][vc_column_text]\n

Training overview<\/h3>\n[\/vc_column_text][vc_column_text]First, a brief repetition of terms from the theory, based on numerous examples, conclusions are drawn for everyday life. Afterwards, we discuss typical reasons for a lack of robustness in the routine with the help of real cases. Many a cause is also mentioned, which one would not think about without further ado. The view is thus also directed to such \u201einconspicuous\u201c things as metal frit, stirrer, plastic attachments, vials, neon light, detergent, degasser, etc. In group work here is learned how to quickly identify causes of error and effectively fixes them. For this we discuss many chromatograms that reflect typical situations. Finally, with the help of self-developed flowcharts, the \u201esymptom-cause\u201c thinking is deliberately practiced for several situations.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_column_text]\n

Training content<\/h3>\n[\/vc_column_text]
\n
    \n
  • Systematic troubleshooting for a change in retention time, peak area, and peak height \u2013 where do I start, where do I stop?<\/li>\n
  • Which problems are common, despite qualified devices and validated methods?<\/li>\n
  • The shape of the peak changes \u2013 is it the pH, the quality of the package or just a single bubble?<\/li>\n
  • Spikes, ghost peaks, noise, baseline fluctuations \u2013 what causes can I exclude directly by looking at the chromatogram?<\/li>\n
  • Which errors are typical for the pump, autosampler, detector?<\/li>\n
  • What errors during sample preparation are noticeable in the chromatogram?<\/li>\n
  • How do I recognize weaknesses in a method intended for transfer?<\/li>\n
  • Suggestions and schemes for the quick limitation of possible causes<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

    Target audience<\/h3>\n[\/vc_column_text]
    \n
      \n
    • HPLC users with many years of practical experience and solid HPLC skills. We strongly recommend the prior attendance of the course \u201eHPLC for advanced users\u201c and\/or \u201eTroubleshooting and Error Prevention in HPLC\u201c<\/li>\n<\/ul>\n <\/div>[divider line_type=”No Line” custom_height=”30″][vc_column_text]\n

      Training objectives<\/h3>\n[\/vc_column_text]
      \n
        \n
      • Fast error recognition even in case of difficult problems and systematic troubleshooting<\/li>\n<\/ul>\n <\/div>[\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

        [vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent”…<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":97,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2701","page","type-page","status-publish"],"yoast_head":"\nAdvanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Advanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas\" \/>\n<meta property=\"og:description\" content=\"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent”...\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/\" \/>\n<meta property=\"og:site_name\" content=\"Dr. Stavros Kromidas\" \/>\n<meta property=\"article:modified_time\" content=\"2023-11-12T09:28:19+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.kromidas.de\/en\/#website\",\"url\":\"https:\/\/www.kromidas.de\/en\/\",\"name\":\"Dr. Stavros Kromidas\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.kromidas.de\/en\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#webpage\",\"url\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/\",\"name\":\"Advanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas\",\"isPartOf\":{\"@id\":\"https:\/\/www.kromidas.de\/en\/#website\"},\"datePublished\":\"2019-05-17T09:59:07+00:00\",\"dateModified\":\"2023-11-12T09:28:19+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"HPLC\",\"item\":\"https:\/\/www.kromidas.de\/en\/hplc\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Courses\",\"item\":\"https:\/\/www.kromidas.de\/en\/hplc\/courses\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Advanced troubleshooting in HPLC \u2013 the tricky cases\"}]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Advanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/","og_locale":"en_US","og_type":"article","og_title":"Advanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas","og_description":"[vc_row type=”full_width_background” full_screen_row_position=”middle” column_margin=”default” scene_position=”center” text_color=”dark” text_align=”left” bottom_padding=”5%” overlay_strength=”0.3″ shape_divider_position=”bottom” bg_image_animation=”none” shape_type=””][vc_column column_padding=”padding-2-percent” column_padding_position=”top” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_link_target=”_self” column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_width_inherit=”default” tablet_text_alignment=”default” phone_text_alignment=”default” overlay_strength=”0.3″ column_border_width=”none” column_border_style=”solid” bg_image_animation=”none”][vc_row_inner column_margin=”default” text_align=”left”][vc_column_inner column_padding=”padding-2-percent”...","og_url":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/","og_site_name":"Dr. Stavros Kromidas","article_modified_time":"2023-11-12T09:28:19+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebSite","@id":"https:\/\/www.kromidas.de\/en\/#website","url":"https:\/\/www.kromidas.de\/en\/","name":"Dr. Stavros Kromidas","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.kromidas.de\/en\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#webpage","url":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/","name":"Advanced troubleshooting in HPLC \u2013 the tricky cases - Dr. Stavros Kromidas","isPartOf":{"@id":"https:\/\/www.kromidas.de\/en\/#website"},"datePublished":"2019-05-17T09:59:07+00:00","dateModified":"2023-11-12T09:28:19+00:00","breadcrumb":{"@id":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/aufbaukurs-fehlersuche-in-der-hplc-fuer-fortgeschrittene-die-kniffligen-faelle\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"HPLC","item":"https:\/\/www.kromidas.de\/en\/hplc\/"},{"@type":"ListItem","position":2,"name":"Courses","item":"https:\/\/www.kromidas.de\/en\/hplc\/courses\/"},{"@type":"ListItem","position":3,"name":"Advanced troubleshooting in HPLC \u2013 the tricky cases"}]}]}},"_links":{"self":[{"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/pages\/2701","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/comments?post=2701"}],"version-history":[{"count":5,"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/pages\/2701\/revisions"}],"predecessor-version":[{"id":4768,"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/pages\/2701\/revisions\/4768"}],"up":[{"embeddable":true,"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/pages\/97"}],"wp:attachment":[{"href":"https:\/\/www.kromidas.de\/en\/wp-json\/wp\/v2\/media?parent=2701"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}