{"id":4312,"date":"2020-02-19T10:30:09","date_gmt":"2020-02-19T09:30:09","guid":{"rendered":"https:\/\/www.kromidas.de\/en\/?page_id=4312"},"modified":"2021-06-14T11:05:51","modified_gmt":"2021-06-14T09:05:51","slug":"hplc-knowledge-compact-2","status":"publish","type":"page","link":"https:\/\/www.kromidas.de\/en\/hplc-knowledge-compact-2\/","title":{"rendered":"HPLC-Knowledge Compact"},"content":{"rendered":"[vc_row type=&#8221;full_width_background&#8221; full_screen_row_position=&#8221;middle&#8221; column_margin=&#8221;default&#8221; scene_position=&#8221;center&#8221; text_color=&#8221;dark&#8221; text_align=&#8221;left&#8221; top_padding=&#8221;3%&#8221; bottom_padding=&#8221;3%&#8221; overlay_strength=&#8221;0.3&#8243; shape_divider_position=&#8221;bottom&#8221; bg_image_animation=&#8221;none&#8221; shape_type=&#8221;&#8221;][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_link_target=&#8221;_self&#8221; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;default&#8221; phone_text_alignment=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;padding-2-percent&#8221; column_padding_position=&#8221;right&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3>\u201eHPLC-Knowledge compact\u201c: Essential Topics condensed taught<\/h3>\n[\/vc_column_text][vc_column_text]In these half-day workshops (8.30-12.00 am and 1.00-4.30 pm) we highlight a specific HPLC-topic very intensive (group work, exercises, question-answer-games). The workshops are suited perfectly for experienced HPLC-users. Different workshops can of course be arbitrary combined, however some topics fit very well together, e.g. in the morning\u00a0<strong>\u201e<\/strong><em><strong>Comparison and selection of HPLC-RP-columns\u201c<\/strong>\u00a0and in the afternoon\u00a0<strong>\u201eWhich gradient should I use?\u201d<\/strong><\/em>\u00a0or in the morning\u00a0<em><strong>\u201eIntelligent method development\u201c<\/strong>\u00a0<\/em>and in the afternoon\u00a0<strong>\u201e<\/strong><em><strong>How to prove robustness\u201c<\/strong>\u00a0<\/em>etc. Participants can in principle attend only the morning-workshop or only the afternoon-workshop as well or of course both.[\/vc_column_text][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row][vc_row type=&#8221;in_container&#8221; full_screen_row_position=&#8221;middle&#8221; column_margin=&#8221;default&#8221; scene_position=&#8221;center&#8221; text_color=&#8221;dark&#8221; text_align=&#8221;left&#8221; overlay_strength=&#8221;0.3&#8243; shape_divider_position=&#8221;bottom&#8221; bg_image_animation=&#8221;none&#8221;][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_link_target=&#8221;_self&#8221; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;default&#8221; phone_text_alignment=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][tabbed_section style=&#8221;vertical&#8221; tab_color=&#8221;Accent-Color&#8221;][tab icon_family=&#8221;none&#8221; title=&#8221;Development of robust HPLC-methods&#8221; id=&#8221;1623661490569-0&#8243; tab_id=&#8221;1623661490571-9&#8243;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3><strong>Development of robust HPLC-methods \u2013 \u201ethat\u00b4s\u201d the way!<\/strong><\/h3>\n[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>Method development under time pressure<\/li>\n<li>Proven\/successful combinations of column\/mobile phase\/pH-value<\/li>\n<li>Tests to prove column stability \u2013 basic requirements for a robust routine method<\/li>\n<\/ul>\n <\/div>[\/vc_column_inner][\/vc_row_inner][\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Tips und hints how to prove the peak-homogeneity&#8221; id=&#8221;1623661490874-3&#8243; tab_id=&#8221;1623661490875-10&#8243;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3><strong>Tips und hints how to prove the peak-homogeneity<\/strong><\/h3>\n[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>Quick tests in absence of LC-MS-coupling<\/li>\n<li>What is \u201ebest\u201c? DAD, LC\/MS\/MS, less injection volume or a polar pre-column?<\/li>\n<li>Tests for the optimum \u201cbenefit-cost-ratio\u201d \u2013 the \u201c5-min method\u201d<\/li>\n<\/ul>\n <\/div>[\/vc_column_inner][\/vc_row_inner][\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Tips for successful method transfers&#8221; id=&#8221;1623661491043-5&#8243; tab_id=&#8221;1623661491044-2&#8243;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3><strong>Tips for successful method transfers<\/strong><\/h3>\n[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>Typical organisational and analytical errors during method transfer<\/li>\n<li>\u201eDo\u00b4s und dont\u00b4s\u201c-list to avoid future trouble<\/li>\n<li>Review of real cases<\/li>\n<\/ul>\n <\/div>[\/vc_column_inner][\/vc_row_inner][\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Testing\/verification of robustness in HPLC&#8221; id=&#8221;1623661491187-0&#8243; tab_id=&#8221;1623661491189-3&#8243;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3><strong>\u201cTesting\u201c\/verification of robustness in HPLC<\/strong><\/h3>\n[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>What is crucial but has been often \u201eforgotten\u201c during testing robustness?<\/li>\n<li>Hints how to realize lack of robustness by carefully reading the method description<\/li>\n<li>Schemes and plans to test robustness dependent to the characteristics of the method and the matrix<\/li>\n<\/ul>\n <\/div>[\/vc_column_inner][\/vc_row_inner][\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Correct integration and correct evaluation of chromatographic data&#8221; id=&#8221;1623661491372-10&#8243; tab_id=&#8221;1623661491373-9&#8243;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;left&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_column_text]\n<h3><strong>Correct integration and correct evaluation of chromatographic data<\/strong><\/h3>\n[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>Poor resolution, \u201esnippy\u201c peaks close to LOQ and\/or drifting baseline \u2013 how to integrate correct, e. g: drop, \u201evaley to valey\u201c, tangential or exponential skim?<\/li>\n<li>Which chromatographic parameters and which software settings affect how strong peak area, peak shape and LOD\/LOQ?<\/li>\n<li>What does mean a \u201etoo\u201c small RSD and what should be the consequence? Is\u00a0<em>this<\/em>\u00a0value a usual deviation or an outlier?<\/li>\n<\/ul>\n <\/div>[\/vc_column_inner][\/vc_row_inner][\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Choosing the \u201eright\u201c eluent&#8221; id=&#8221;1623661491490-7&#8243; tab_id=&#8221;1623661491491-0&#8243;][vc_column_text]<strong>Choosing the \u201eright\u201c eluent \u2013 incl. gradient elution <\/strong>(mobile phase in detail\u00a0plus a little bit gradient)[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>How to quickly find the appropriate composition of the mobile phase and of the right pH-value dependent on the analyte or the real aim of the method?<\/li>\n<li>When methanol, when acetonitril, when, which pH-value (<em>and<\/em>\u00a0buffer)?<\/li>\n<li>Exercises for optimization and troubleshooting of gradient runs<\/li>\n<\/ul>\n <\/div>[\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;HPLC in pharmaceutical companies&#8221; id=&#8221;1623661491574-0&#8243; tab_id=&#8221;1623661491575-5&#8243;][vc_column_text]<strong>HPLC in pharmaceutical companies; specific requirements und tips for the daily work<\/strong>[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>System Suitability Test, SST; what, how, how often \u2013 requirements und reality<\/li>\n<li>Which guidelines are clear and which are \u201cinterpretable\u201d? News and trends by the FDA, USP, ICH, Pharm.Eur.<\/li>\n<li>Which claims are for the authorities very important, where lies the emphasis by the tests?<\/li>\n<\/ul>\n <\/div>[\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Such a way you get the optimal output from your HPLC\/UHPLC&#8221; id=&#8221;1623661491651-6&#8243; tab_id=&#8221;1623661491652-4&#8243;][vc_column_text]<strong>Such a way you get the optimal output from your HPLC\/UHPLC<\/strong>[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>HPLC vs. UHPLC: Benefits and drawbacks in the daily work<\/li>\n<li>Why do some modern columns in modern equipments sow a bad performance? Typical hardware-pitfalls<\/li>\n<li>Pro\u00b4s and Con\u00b4s of binary and ternary pumps, pre-heater or heat exchanger capillary etc. What are really the major\/particular differences of modern HPLC\/UHPLC-equipment?<\/li>\n<\/ul>\n <\/div>[\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;Typical errors during sample separation&#8221; id=&#8221;1623661491806-4&#8243; tab_id=&#8221;1623661491807-5&#8243;][vc_column_text]<strong>Typical errors during sample separation<\/strong>[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>Lost of analyte during the sample preparation procedure \u2013 the main reasons<\/li>\n<li>Kind of diluent and solving handling\u00a0 \u2013 their influence on peak shape, number of peaks and the reproducibility of the peak area<\/li>\n<li>How to avoid errors by preparing the mobile phase<\/li>\n<\/ul>\n <\/div>[\/tab][tab icon_family=&#8221;none&#8221; title=&#8221;A special, tailor-made workshop!&#8221; id=&#8221;1623661491898-3&#8243; tab_id=&#8221;1623661491900-4&#8243;][vc_column_text]<strong><em>Your<\/em><\/strong><strong>\u00a0HPLC-method(s) in the focus of your in-house-training!<\/strong><\/p>\n<p>We prepare a custom-made workshop in which your methods are in focus \u2013 up to 4 methods per \u00bd -day workshop. Considering your whishes in the training we focus on sample preparation (method A), or\/and optimization (method B), or\/and integration (method C)\u2026We support you, so\u00a0<em>that<\/em>\u00a0method becomes more robust,\u00a0<em>this\u00a0<\/em>one faster and in the third case you can save a lot of mobile phase by changing just few things.<\/p>\n<p>The progress of the workshop is really individual, this particular training is based on your aim, e. g:[\/vc_column_text]<div class=\"nectar-fancy-ul\" data-list-icon=\"icon-salient-thin-line\" data-animation=\"false\" data-animation-delay=\"0\" data-color=\"accent-color\" data-spacing=\"default\" data-alignment=\"left\"> \n<ul>\n<li>You\u00b4d like to come to optimized methods \u2013 incl. hand-on in\u00a0<em>your<\/em>\u00a0lab at\u00a0<em>your<\/em>\u00a0HPLC-equipment? We work with you in your lab!<\/li>\n<li>You\u00b4d like to convert a 30-40 min. method to a in 5-10 min robust method with no or litlle revalidation work? Do you like to use your UHPLC really as \u201eUHPLC\u201c?<\/li>\n<li>You\u00b4d like to learn how to find an alternative, more robust column for\u00a0<em>these\u00a0<\/em>particular compounds? We bring a numerous of columns to try them out with you in your lab.<\/li>\n<\/ul>\n <\/div>[\/tab][\/tabbed_section][\/vc_column][\/vc_row][vc_row type=&#8221;full_width_background&#8221; full_screen_row_position=&#8221;middle&#8221; equal_height=&#8221;yes&#8221; content_placement=&#8221;middle&#8221; column_margin=&#8221;default&#8221; bg_color=&#8221;#ee9d34&#8243; scene_position=&#8221;center&#8221; text_color=&#8221;light&#8221; text_align=&#8221;left&#8221; top_padding=&#8221;40&#8243; bottom_padding=&#8221;40&#8243; overlay_strength=&#8221;0.3&#8243; shape_divider_position=&#8221;bottom&#8221; bg_image_animation=&#8221;none&#8221; shape_type=&#8221;&#8221;][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_link_target=&#8221;_self&#8221; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; width=&#8221;1\/2&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;center&#8221; phone_text_alignment=&#8221;center&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_custom_heading text=&#8221;Find the best training concept for you&#8221; font_container=&#8221;tag:h3|font_size:52|text_align:left|line_height:56px&#8221; use_theme_fonts=&#8221;yes&#8221; css=&#8221;.vc_custom_1582105836089{padding-left: 25px !important;}&#8221;][\/vc_column][vc_column column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_link_target=&#8221;_self&#8221; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; width=&#8221;1\/2&#8243; tablet_width_inherit=&#8221;default&#8221; tablet_text_alignment=&#8221;center&#8221; phone_text_alignment=&#8221;center&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][vc_row_inner column_margin=&#8221;default&#8221; text_align=&#8221;right&#8221; css=&#8221;.vc_custom_1550215597768{padding-right: 25px !important;}&#8221;][vc_column_inner column_padding=&#8221;no-extra-padding&#8221; column_padding_position=&#8221;all&#8221; background_color_opacity=&#8221;1&#8243; background_hover_color_opacity=&#8221;1&#8243; column_shadow=&#8221;none&#8221; column_border_radius=&#8221;none&#8221; column_link_target=&#8221;_self&#8221; width=&#8221;1\/1&#8243; tablet_width_inherit=&#8221;default&#8221; overlay_strength=&#8221;0.3&#8243; column_border_width=&#8221;none&#8221; column_border_style=&#8221;solid&#8221; bg_image_animation=&#8221;none&#8221;][nectar_btn size=&#8221;large&#8221; button_style=&#8221;see-through-3d&#8221; color_override=&#8221;#ffffff&#8221; url=&#8221;https:\/\/www.kromidas.de\/en\/kontakt\/&#8221; text=&#8221;contact me&#8221;][\/vc_column_inner][\/vc_row_inner][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"<p>[vc_row type=&#8221;full_width_background&#8221; 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